Speaker

Title

Phosphoproteomic landscapes of exercise to identify functional phosphorylation

Abstract

Phosphoproteomics has revolutionised our ability to rapidly quantify thousands of phosphorylation sites. Here, we present our phosphoproteome integrations of various exercise modalities in human skeletal muscle with cell- and rodent-based models to identify functionally relevant phosphorylation. We further profiled various exercise mimetics in vitro and identified direct kinase:substrate relationships via whole cell lysate in vitro kinase assays. Finally, we identify exercise-regulated phosphorylation sites that modulate in vivo skeletal muscle function and promote protein:protein interactions via mutational analysis.

A/Prof Benjamin Parker  was trained in pharmacology and mass spectrometry in the Australian Sports Drug Testing Laboratory. He completed is PhD at the University of Southern Denmark and University of Sydney where he developed assays to quantify protein modifications by mass spectrometry applied to study cardiovascular disease. He performed post-doctoral research at the Garvan Institute and then obtained an NHMRC Fellowship in the Metabolic Systems Biology Program at the Charles Perkins Centre. He is currently an NHMRC Investigator and group leader at The University of Melbourne. His research team is focused on understanding how genetic variants and signal transduction regulate metabolism and musculoskeletal health with the goal of identifying new therapeutic targets to treat metabolic disease.

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